RCAS-RNAi: a loss-of-function method for the developing chick retina.

Sanjiv Harpavat; Constance L Cepko

doi:10.1186/1471-213x-6-2

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RCAS-RNAi: a loss-of-function method for the developing chick retina.

Sanjiv Harpavat·Constance L Cepko

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Abstract

BACKGROUND: The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function. RESULTS: We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick eye. These viruses 'knock down' specific genes in infected areas of the retina. The knock down persists as the retina matures and can be detected using in situ hybridization. Furthermore, the amount of retinal tissue affected can be controlled by manipulating the degree of infection. CONCLUSION: This technique provides a rapid and efficient loss-of-function option for studies in the developing chick retina.

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Animals Base Sequence Chick Embryo Chickens Gene Expression Regulation, Developmental Iodide Peroxidase Mice Molecular Sequence Data Nerve Tissue Proteins Organ Specificity RNA Interference RNA, Messenger Retina Retroviridae Iodothyronine Deiodinase Type II Journal Article Research Support, Non-U.S. Gov't

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Sanjiv Harpavat, Constance L Cepko. 2006-01-22. RCAS-RNAi: a loss-of-function method for the developing chick retina.. https://doi.org/10.1186/1471-213x-6-2

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