Discontinuous epitope prediction based on mimotope analysis.

Violaine Moreau; Claude Granier; Sylvie Villard; Daniel Laune; Franck Molina

doi:10.1093/bioinformatics/btl012

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Discontinuous epitope prediction based on mimotope analysis.

Violaine Moreau·Claude Granier·Sylvie Villard·Daniel Laune·Franck Molina

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Abstract

MOTIVATION: Phage display is a widespread technique used to obtain peptide mimotopes selected by binding to a given monoclonal antibody in a similar way as the native epitope. However, the localization of the interaction site mimicked by the mimotopes on the surface of the antigen is not always a straightforward task. MIMOP is a computational tool developed with the aim of helping experimentalists to analyze a set of mimotope sequences and guide them in the identification of the mimicked region. RESULTS: To predict potential epitopic regions, MIMOP integrates two different approaches combining two- and three-dimensional analyses: MimAlign starts from degenerated alignment analyses, and MimCons is based on consensus identification. The relevance and usefulness of the tool are illustrated by four use cases corresponding to real-life situations.

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Algorithms Amino Acid Sequence Binding Sites Epitope Mapping Epitopes Molecular Sequence Data Protein Binding Sequence Alignment Sequence Analysis, Protein Journal Article

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BibTeX RIS

Violaine Moreau, Claude Granier, Sylvie Villard, Daniel Laune, Franck Molina. 2006-01-24. Discontinuous epitope prediction based on mimotope analysis.. https://doi.org/10.1093/bioinformatics%2Fbtl012

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