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PubMed · 1644310

Plasmids with easily excisable xylE cassettes.

D C Stein
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Abstract

Two new vectors containing the xylE gene (encoding catechol-2,3-dioxygenase) of Pseudomonas putida were constructed that serve as the source of the xylE cassette. These vectors are based on the kanamycin-resistance-encoding plasmid, pKAN18. The promoter-less xylE gene is flanked by several restriction enzyme sites that allow for easy excision of this gene in the form of a cassette containing a ribosome-binding site, 7 bp upstream from the start codon. These cassettes lack any transcriptional termination signals downstream from the stop codon.

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Base SequenceCatechol 2,3-DioxygenaseCloning, MolecularDNA, BacterialDioxygenasesMolecular Sequence DataOxygenasesPlasmidsPseudomonas putidaRestriction MappingJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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BibTeXRIS

D C Stein. 1992-08-01. Plasmids with easily excisable xylE cassettes.. https://doi.org/10.1016/0378-1119(92)90506-k

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