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PubMed · 16443818

Cryo-EM and single particles.

Liguo Wang·Fred J Sigworth
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Abstract

Cryoelectronmicroscopy is a method for the imaging of macromolecules in the electron microscope. It was originally developed to determine membrane protein structures from two-dimensional crystals, but more recently "single-particle" techniques have become powerful and popular. Three-dimensional reconstructions are obtained from sets of single-particle images by extensive computer processing; the methods are being applied to many macromolecular assemblies.

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AnimalsCell MembraneCryoelectron MicroscopyCrystallographyHumansImage Processing, Computer-AssistedImaging, Three-DimensionalMacromolecular SubstancesRyanodine Receptor Calcium Release ChannelJournal ArticleResearch Support, N.I.H., ExtramuralReview

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Liguo Wang, Fred J Sigworth. 2006. Cryo-EM and single particles.. https://doi.org/10.1152/physiol.00045.2005

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