Parallel picoliter rt-PCR assays using microfluidics.

Joshua S Marcus; W French Anderson; Stephen R Quake

doi:10.1021/ac0513865

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Parallel picoliter rt-PCR assays using microfluidics.

Joshua S Marcus·W French Anderson·Stephen R Quake

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Abstract

The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis.

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Humans Male Microfluidic Analytical Techniques Microfluidics RNA Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity Journal Article Research Support, N.I.H., Extramural

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Joshua S Marcus, W French Anderson, Stephen R Quake. 2006-02-01. Parallel picoliter rt-PCR assays using microfluidics.. https://doi.org/10.1021/ac0513865

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