Multiple ligand applications in high-performance immunoaffinity chromatography.

J B Wheatley

doi:10.1016/0021-9673(92)85371-y

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Multiple ligand applications in high-performance immunoaffinity chromatography.

J B Wheatley

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Abstract

A mixture of antibodies specific for albumin and transferrin, immobilized onto a single support, was used for the simultaneous extraction of albumin and transferrin from immunoglobulin G by high-performance immunoaffinity chromatography. The affinity column was coupled to a strong cation exchanger in order to monitor the success of the extraction and to demonstrate the compatibility of the two chromatographic modes. Coupling of non-affinity chromatography with multiple ligand affinity chromatography is discussed as an alternative to positive affinity chromatography for protein purification.

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Chromatography, Affinity Chromatography, Ion Exchange Humans Immune Sera Immunoglobulin G Ligands Serum Albumin Spectrophotometry, Ultraviolet Transferrin Journal Article

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J B Wheatley. 1992-06-19. Multiple ligand applications in high-performance immunoaffinity chromatography.. https://doi.org/10.1016/0021-9673(92)85371-y

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