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Solid-phase enzyme-linked immunosorbent assay for hepatitis E virus IgG and IgM antibodies utilizing recombinant antigens and synthetic peptides.

G J Dawson·K H Chau·C M Cabal·P O Yarbough·G R Reyes·I K Mushahwar
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Abstract

Four recombinant antigens representing two distinct antigenic domains from two different strains of hepatitis E virus (HEV), were used individually to develop four ELISAs designed to detect antibodies to HEV. Both IgG and IgM class antibodies to HEV were detected in 7 of 8 pedigreed serum/plasma from known outbreaks of HEV in Mexico, Burma, Somalia and Pakistan. In addition, specific HEV-antibodies were detected in cynomolgus macaques following inoculation with various HEV strains. Anti-HEV was also detected in 8 of 386 (2.1%) randomly selected American blood donors. Supplemental tests utilizing both synthetic peptides and specific blocking assays provided additional serologic data confirming the presence of anti-HEV. Similar prevalence studies on a limited number of available sera from other geographical regions (Alaska, Japan, Germany, New Zealand, Thailand and Mexico) confirmed the presence of anti-HEV in at least 1.1 to 7.6% of the specimens.

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AnimalsAntigens, ViralEnzyme-Linked Immunosorbent AssayHepatitis AntibodiesHepatitis EHepatitis E virusHumansImmunoglobulin GImmunoglobulin MMacaca fascicularisPeptidesPrevalenceRecombinant ProteinsSensitivity and SpecificitySeroepidemiologic StudiesJournal Article

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G J Dawson, K H Chau, C M Cabal, P O Yarbough, G R Reyes, I K Mushahwar. 1992. Solid-phase enzyme-linked immunosorbent assay for hepatitis E virus IgG and IgM antibodies utilizing recombinant antigens and synthetic peptides.. https://doi.org/10.1016/0166-0934(92)90180-l

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