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Biomolecular interaction analysis under electrophoretic flow conditions.

Michael Kumpf·Günter Gauglitz
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Abstract

Combining the advantages of electrophoresis with the advantages of biomolecular interaction analysis (BIA) enables the biospecific detection of separated molecules; for example it permits differentiation between a complementary single-stranded DNA and a single nucleotide polymorphism. In order to integrate these two techniques, it is necessary to investigate whether it is possible to detect a biomolecular interaction under electrophoretic flow conditions. To this end a novel detection system was developed for electrophoresis that utilizes a label-free and time-resolved detection technique: reflectometric interference spectroscopy (RIfS). The biological functions of important analytes were investigated using this system. Although RIfS can be used as a postcolumn detector, it is also possible to use it to detect relevant substances under electrophoretic flow conditions. DNA-LNA, biotin-streptavidin and protein-protein interactions were detected using this coupled electrophoresis-RIfS set-up.

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AntibodiesBiotinDNAElectrophoresisMicroscopy, InterferenceOligonucleotidesOligonucleotides, AntisensePolymorphism, Single NucleotideSensitivity and SpecificityStreptavidinTime FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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Michael Kumpf, Günter Gauglitz. 2006-02-08. Biomolecular interaction analysis under electrophoretic flow conditions.. https://doi.org/10.1007/s00216-005-0283-3

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