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PubMed · 16468354

[Study on rhG-CSF modified with polyethylene glycol].

Lin-Lin Zhang·Chun-Yang Zheng·Jian-Du Lei·Guang-Hui Ma·Zhi-Guo Su·Li Wang
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Abstract

Monomethoxy Polyethylene Glycol(mPEG20000) was activated by N-hydroxysuccinimede and analyzed by infrared spectrum and hydrolysis kinetics. In order to propose the optimized reaction conditions of mono-PEGylated rhG-CSF, orthogonal design of the experiment was investigated. Ion exchange chromatography was used to separate and purify PEGylated rhG-CSF from unPEGylated rhG-CSF. The purity of mono-PEGylated rhG-CSF was analyzed by high performance liquid chromatography (HPLC) to be 97%.

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Granulocyte Colony-Stimulating FactorHumansPolyethylene GlycolsRecombinant ProteinsSurface PropertiesJournal ArticleResearch Support, Non-U.S. Gov't

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Lin-Lin Zhang, Chun-Yang Zheng, Jian-Du Lei, Guang-Hui Ma, Zhi-Guo Su, Li Wang. 2005. [Study on rhG-CSF modified with polyethylene glycol].. https://pubmed.ncbi.nlm.nih.gov/16468354/

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