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PubMed · 16472693

Tumor cell migration in three dimensions.

Steven Hooper·John F Marshall·Erik Sahai
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Abstract

In almost all physiological and pathological situations, cells migrate through three-dimensional environments, yet most studies of cell motility have used two-dimensional substrates. It is clear that two-dimensional substrates do not mimic the in vivo environment accurately, and recent work using three-dimensional environments has revealed many different mechanisms of cell migration (Abbott, 2003; Sahai and Marshall, 2003; Wolf et al., 2003). This chapter will describe methods for generating three-dimensional matrices suitable for studying cell motility, methods for imaging the morphology of motile cells in situ, and methods for quantifying cell migration through three-dimensional environments.

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AmidesAnimalsCarcinoma, Squamous CellCell Culture TechniquesCell LineCell Line, TumorCell MovementCollagenCollagen Type IDrug CombinationsExtracellular MatrixHumansIntracellular Signaling Peptides and ProteinsLamininMelanomaMicroscopy, ConfocalMicroscopy, Electron, ScanningProtein Serine-Threonine KinasesProteoglycansPyridinesrho-Associated KinasesComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Steven Hooper, John F Marshall, Erik Sahai. 2006. Tumor cell migration in three dimensions.. https://doi.org/10.1016/s0076-6879(06)06049-6

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