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Using three-dimensional acinar structures for molecular and cell biological assays.

Bin Xiang·Senthil K Muthuswamy
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Abstract

Epithelial cells grown in three-dimensional matrix allows one to investigate biological processes in a setting that is closer to in vivo conditions than those obtained by growing cells on plastic culture dishes. Here we outline procedures that allow one to investigate molecular mechanisms that regulate formation and disruption of three-dimensional epithelial structures.

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Cell Culture TechniquesCollagenCyclin-Dependent Kinase 2Cyclin-Dependent Kinase 4Drug CombinationsEpithelial CellsFluorescent Antibody Technique, IndirectHumansLamininProteoglycansJournal Article

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Bin Xiang, Senthil K Muthuswamy. 2006. Using three-dimensional acinar structures for molecular and cell biological assays.. https://doi.org/10.1016/s0076-6879(06)06054-x

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