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Selective MCA occlusion: a precise embolic stroke model.

Vincent A Dinapoli·Charles L Rosen·Tomoaki Nagamine·Todd Crocco
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Abstract

The present study describes a method for improving the precision and accuracy of clot placement within the middle cerebral artery (MCA) of rats, utilizing a micro-catheter and laser Doppler flowmetry. This technique reduces the size of clot needed to achieve stable occlusion with no failed embolizations and a low percentage of early recanalizations. Infarctions were consistent in both size and distribution within the MCA perfusion territory. Selective embolization in aged animals (n = 10) resulted in substantially larger infarctions than those seen in aged animals (n = 10) following non-selective embolization (P < 0.05), or young animals (n = 10) subjected to filamentous occlusion (P < 0.001). Clots were localized to the MCA by direct examination at 0, 60 and 120 min post-embolization (n = 14). All aged animals surviving 24h exhibited moderate to severe functional deficits, with selectively occluded animals having a higher mean score on the modified neurologic severity scale (P = 0.002). This model provides a highly reproducible method for embolization of the MCA and reliable reperfusion with rt-PA.

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AgingAnimalsBlood PressureBrain IschemiaCarbon DioxideCarotid ArteriesCerebral InfarctionDisease Models, AnimalFemaleHematocritHydrogen-Ion ConcentrationIntracranial EmbolismMiddle Cerebral ArteryPlasminogen ActivatorsRatsRats, Sprague-DawleyRecombinant ProteinsStrokeThrombolytic TherapyUltrasonography, Doppler, TranscranialJournal Article

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Vincent A Dinapoli, Charles L Rosen, Tomoaki Nagamine, Todd Crocco. 2006-02-10. Selective MCA occlusion: a precise embolic stroke model.. https://doi.org/10.1016/j.jneumeth.2005.12.026

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