A stability-indicating HPLC assay method for docetaxel.

B Mallikarjuna Rao; Arpita Chakraborty; M K Srinivasu; M Lalitha Devi; P Rajender Kumar; K B Chandrasekhar; A K Srinivasan; A S Prasad; J Ramanatham

doi:10.1016/j.jpba.2006.01.011

PubMed · 16473490

A stability-indicating HPLC assay method for docetaxel.

B Mallikarjuna Rao·Arpita Chakraborty·M K Srinivasu·M Lalitha Devi·P Rajender Kumar·K B Chandrasekhar·A K Srinivasan·A S Prasad·J Ramanatham

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Abstract

A novel stability-indicating high-performance liquid chromatographic assay method was developed and validated for docetaxel in the presence of degradation products generated from forced decomposition studies. A gradient HPLC method was developed to separate the drug from the degradation products, using a Hichrom RPB HPLC column. Mixture of water and acetonitrile was used as mobile phase. The flow rate was 1.0 ml/min and the detection was done at 230 nm. Using the above method one can carry out the quantitative estimation of impurity namely DCT-1 and docetaxel. The developed gradient LC method was subsequently validated.

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Antineoplastic Agents, Phytogenic Chromatography, High Pressure Liquid Docetaxel Drug Stability Reproducibility of Results Taxoids Technology, Pharmaceutical Journal Article Research Support, Non-U.S. Gov't

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B Mallikarjuna Rao, Arpita Chakraborty, M K Srinivasu, M Lalitha Devi, P Rajender Kumar, K B Chandrasekhar, A K Srinivasan, A S Prasad, J Ramanatham. 2006-02-13. A stability-indicating HPLC assay method for docetaxel.. https://doi.org/10.1016/j.jpba.2006.01.011

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