Microfluorometry of cell membrane dynamics.

Petra Weber; Michael Wagner; Herbert Schneckenburger

doi:10.1002/cyto.a.20233

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Microfluorometry of cell membrane dynamics.

Petra Weber·Michael Wagner·Herbert Schneckenburger

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Abstract

Membranes of living cells are characterized by a combination of conventional and total internal reflection fluorescence microscopy (TIRFM) using the membrane marker laurdan. In the first case, all cellular membranes are assessed simultaneously, whereas in the second case, the plasma membrane is excited selectively by the evanescent electromagnetic field of a laser beam. A spectral shift depending on the phase of membrane lipids is used to characterize membrane stiffness, which decreases with temperature and increases with the amount of cholesterol. Spectral properties are evaluated and displayed as microscopic images.

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2-Naphthylamine Cell Line, Tumor Cell Membrane Cholesterol Cytophotometry Fluorescence Polarization Fluorescent Dyes Humans Intracellular Membranes Laurates Membrane Fluidity Membrane Lipids Spectrometry, Fluorescence beta-Cyclodextrins Journal Article Research Support, Non-U.S. Gov't

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Petra Weber, Michael Wagner, Herbert Schneckenburger. 2006. Microfluorometry of cell membrane dynamics.. https://doi.org/10.1002/cyto.a.20233

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