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An improved method for discrimination of cell populations in tissue sections using microscopy-based multicolor tissue cytometry.

Rupert C Ecker·Radu Rogojanu·Marc Streit·Katja Oesterreicher·Georg E Steiner
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Abstract

BACKGROUND: In tissue context, researchers and pathologists lack a generally applicable standard for quantitative determination of cytological parameters. Increasing knowledge of disease-specific markers calls for an appropriate in situ tissue cytometry. METHODS: Microscopy-based multicolor tissue cytometry (MMTC) permits multicolor analysis of single cells within tissue context. RESULTS: Tissue specimens stained for CD45/CD3/CD4/CD8 were analyzed. Specificity as well as reproducibility of MMTC is demonstrated and a novel MMTC-based function to improve visual discrimination of subpopulations is introduced. CONCLUSIONS: Our data demonstrate that MMTC constitutes an important step toward automated and quantitative fluorometry of solid tissues and cell monolayers.

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AlgorithmsAntigens, CDEukaryotic CellsHumansImage CytometryImage Processing, Computer-AssistedLaser Scanning CytometryLeukocyte Common AntigensLeukocytesLeukocytes, MononuclearReproducibility of ResultsStaining and LabelingTissue FixationJournal Article

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Rupert C Ecker, Radu Rogojanu, Marc Streit, Katja Oesterreicher, Georg E Steiner. 2006. An improved method for discrimination of cell populations in tissue sections using microscopy-based multicolor tissue cytometry.. https://doi.org/10.1002/cyto.a.20219

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