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A resazurin-based biosensor for organic pollutants.

Aynsley C Tizzard·Joost H Bergsma·Gareth Lloyd-Jones
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Abstract

A new rapid biosensor method employing the dye resazurin as an indicator of bacterial respiration has been developed to provide a rapid, facile and specific biosensor for environmental contaminants that does not rely on genetic modification techniques, is suitable for a high-throughput multiwell format, and is ideally suited to resource-constrained environmental monitoring situations. This whole-cell biosensor has been applied to the test analyte toluene using natural toluene-degrading bacteria as the biological component and is competitive with more complex recombinant approaches. The redox-driven biosensor is dependent on the catabolism of a specific compound, concomitantly reducing the redox indicator resazurin to provide the analytical signal in a whole-cell biosensor assay.

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Biological AssayBiosensing TechniquesBurkholderia cepaciaDose-Response Relationship, DrugEnvironmental MonitoringEnvironmental PollutantsOrganic ChemicalsOxazinesPseudomonasTolueneXanthenesJournal ArticleResearch Support, Non-U.S. Gov't

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Aynsley C Tizzard, Joost H Bergsma, Gareth Lloyd-Jones. 2006-02-17. A resazurin-based biosensor for organic pollutants.. https://doi.org/10.1016/j.bios.2006.01.011

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