Sequencing and staphylococci identification.

Alexander Mellmann; Karsten Becker; Christof von Eiff; Ursula Keckevoet; Peter Schumann; Dag Harmsen

doi:10.3201/eid1202.050962

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Sequencing and staphylococci identification.

Alexander Mellmann·Karsten Becker·Christof von Eiff·Ursula Keckevoet·Peter Schumann·Dag Harmsen

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Abstract

The emerging clinical importance of staphylococcal infections prompted us to establish a reference database for partial RNA polymerase B (rpoB; nucleotides 1444-1928) gene sequences from type strains of all staphylococcal species and subspecies. This database correctly identified 55 clinical staphylococcal isolates; all were correctly identified at the species level. At the subspecies level, rpoB misidentified only 2 isolates.

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Animals Bacterial Typing Techniques Databases, Genetic Humans RNA Polymerase II Reference Standards Sequence Analysis, DNA Species Specificity Staphylococcal Infections Staphylococcus Evaluation Study Journal Article

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Alexander Mellmann, Karsten Becker, Christof von Eiff, Ursula Keckevoet, Peter Schumann, Dag Harmsen. 2006. Sequencing and staphylococci identification.. https://doi.org/10.3201/eid1202.050962

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