A transfer RNA-dependent protein synthesizing system from Ehrlich ascites extracts.

O K Sharma; W K Roberts; D N Beezley; E Borek

doi:10.1016/0005-2787(75)90353-6

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A transfer RNA-dependent protein synthesizing system from Ehrlich ascites extracts.

O K Sharma·W K Roberts·D N Beezley·E Borek

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Abstract

An homologous cell-free system from Ehrlich ascites cells was made tRNA dependent by passing both the ribosomes and the ammonium sulfate fraction through DEAE-cellulose columns in 0.30 M KCl. Protein synthesis was routinely stimulated 20-50 fold by the addition of 100 mug/ml of ascites cell tRNA in the presence of oviduct mRNA, ascites mRNA, or encephalomyocarditis RNA.

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Animals Carcinoma, Ehrlich Tumor Cell Fractionation Chromatography, DEAE-Cellulose Encephalomyocarditis virus Kinetics Mice Neoplasm Proteins Oviducts Protein Biosynthesis RNA, Messenger RNA, Neoplasm RNA, Transfer RNA, Viral Ribosomes Journal Article Research Support, U.S. Gov't, P.H.S.

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O K Sharma, W K Roberts, D N Beezley, E Borek. 1975-05-16. A transfer RNA-dependent protein synthesizing system from Ehrlich ascites extracts.. https://doi.org/10.1016/0005-2787(75)90353-6

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