Single-exposure optical sectioning by color structured illumination microscopy.

Leo G Krzewina; Myung K Kim

doi:10.1364/ol.31.000477

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Single-exposure optical sectioning by color structured illumination microscopy.

Leo G Krzewina·Myung K Kim

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Abstract

Structured illumination microscopy (SIM) is a wide-field technique that rivals confocal microscopy in optical sectioning ability at a small fraction of the acquisition time. For standard detectors such as a CCD camera, SIM requires a minimum of three sequential frame captures, limiting its usefulness to static objects. By using a color grid and camera, we surpass this limit and achieve optical sectioning with just a single image acquisition. The extended method is now applicable to moving objects and improves the speed of three-dimensional imaging of static objects by at least a factor of three.

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Leo G Krzewina, Myung K Kim. 2006-02-15. Single-exposure optical sectioning by color structured illumination microscopy.. https://doi.org/10.1364/ol.31.000477

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