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Recognition imaging with a DNA aptamer.

Liyun Lin·Hongda Wang·Yan Liu·Hao Yan·Stuart Lindsay
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Abstract

We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (approximately 90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.

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Aluminum SilicatesAptamers, NucleotideDNAHumansImmunoglobulin EMicroscopy, Atomic ForceProtein BindingThrombinJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, U.S. Gov't, Non-P.H.S.

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Liyun Lin, Hongda Wang, Yan Liu, Hao Yan, Stuart Lindsay. 2006-03-02. Recognition imaging with a DNA aptamer.. https://doi.org/10.1529/biophysj.105.079111

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