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F0F1-ATPase as biosensor to detect single virus.

Xiaolong Liu·Yun Zhang·Jiachang Yue·Peidong Jiang·Zhenxi Zhang
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Abstract

F(0)F(1)-ATPase within chromatophore was constructed as a biosensor (immuno-rotary biosensor) for the purpose of capturing single virus. Capture of virus was based on antibody-antigen reaction. The detection of virus based on proton flux change driven by ATP-synthesis of F(0)F(1)-ATPase, which was indicated by F1300, was directly observed by a fluorescence microscope. The results demonstrate that the biosensor loading of virus particles has remarkable signal-to-noise ratio (3.8:1) compared to its control at single molecular level, and will be convenient, quick, and even super-sensitive for detecting virus particles.

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Biosensing TechniquesEnzyme-Linked Immunosorbent AssayImmunoassayInfluenza A virusMicroscopy, FluorescenceProton-Translocating ATPasesReproducibility of ResultsSensitivity and SpecificitySpectrometry, FluorescenceEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Xiaolong Liu, Yun Zhang, Jiachang Yue, Peidong Jiang, Zhenxi Zhang. 2006-02-28. F0F1-ATPase as biosensor to detect single virus.. https://doi.org/10.1016/j.bbrc.2006.02.103

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