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A simple method for constructing a tagged protein.

C Jo·J Kim·S A Jo
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Abstract

We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.

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AnimalsBase SequenceCell LineGenetic EngineeringMiceMolecular Sequence DataRecombinant Fusion ProteinsSUMO-1 ProteinJournal ArticleResearch Support, Non-U.S. Gov't

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C Jo, J Kim, S A Jo. 2005. A simple method for constructing a tagged protein.. https://doi.org/10.2174/0929866054696064

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