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PubMed · 16524184

[Constructing of STR slippage model by optimizing some factors].

Jian-qiang Deng·Ying-bi Li·Jin Wu·Yi-ping Hou
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Abstract

OBJECTIVE: To construct STR slippage model and study factors involved in this procedure. METHODS: DNA samples were amplified with the technology of Degenerate oligonucleotide- primed PCR, then their products were taken as later DNA template and their STR genotype were analyzed by optimizing several factors. RESULTS: STR slippage model was constructed. CONCLUSION: Several factors were involved in the produce of STR slippage, such as amount of modulate DNA, concentration of MgCl2, property of DNA polymerase, motif sequence of STR loci, sample, etc.

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DNADNA PrimersGenetic MarkersGenetic VariationGenotypeHumansMagnesiumModels, GeneticPolymerase Chain ReactionTandem Repeat SequencesEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Jian-qiang Deng, Ying-bi Li, Jin Wu, Yi-ping Hou. 2006. [Constructing of STR slippage model by optimizing some factors].. https://pubmed.ncbi.nlm.nih.gov/16524184/

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