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Extracellular matrix alterations in conventional renal cell carcinomas by tissue microarray profiling influenced by the persistent, long-term, low-dose ionizing radiation exposure in humans.

Alina Romanenko·Luisa Morell-Quadreny·David Ramos·Valentin Nepomnyaschiy·Alexander Vozianov·Antonio Llombart-Bosch
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Abstract

The present study was carried out in order to examine molecular alterations of extracellular matrix (ECM), associated with cell-cell communication in conventional (clear-cell) renal cell carcinomas (cRCCs) influenced by persistent long-term, low-dose ionizing radiation (IR) exposure to patients living more than 19 years after the Chernobyl accident in Cesium 137 (137Cs)-contaminated areas of Ukraine. The ECM major components such as fibronectin, laminin, E-cadherin/beta-catenin complexes and p53 tumor suppressor gene protein, and transforming growth factor beta 1 (TGF-beta1) were immunohistochemically (IHC) evaluated in cRCCs from 59 Ukrainian patients, which represented 18 patients living in non-contaminated areas and 41 patients from 137Cs-contaminated areas. In contrast, a control group of 19 Spanish patients with analogue tumors were also investigated. For IHC evaluation, a tissue microarray technique was used. Decrease or loss and abnormal distribution of fibronectin, laminin, E-cadherin/beta-catenin complexes accompanied by elevated levels of p53 and TGF-beta1 were detected in the Ukrainian cRCCs from 137Cs-contaminated areas with statistically significant differences. Thus, our study suggests that chronic long-term, low-dose IR exposure might result in global remodeling of ECM components of the cRCCs with disruption in peri-epithelial stroma and epithelial basement membranes.

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AdolescentAdultAgedAged, 80 and overCadherinsCarcinoma, Renal CellChernobyl Nuclear AccidentExtracellular MatrixFemaleFibronectinsGene ExpressionHumansImmunohistochemistryKidney NeoplasmsLamininMaleMiddle AgedNeoplasm StagingRadiation EffectsTime FactorsTransforming Growth Factor betaTumor Suppressor Protein p53Journal ArticleResearch Support, Non-U.S. Gov't

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Alina Romanenko, Luisa Morell-Quadreny, David Ramos, Valentin Nepomnyaschiy, Alexander Vozianov, Antonio Llombart-Bosch. 2006-03-09. Extracellular matrix alterations in conventional renal cell carcinomas by tissue microarray profiling influenced by the persistent, long-term, low-dose ionizing radiation exposure in humans.. https://doi.org/10.1007/s00428-006-0160-2

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