PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 16533918

Molecular crowding effects on protein stability.

Florin Despa·Dennis P Orgill·Raphael C Lee
View original source ↗Export metadata ↓

Abstract

The volume fraction occupied by the dry matter of the cell can be as large as 40%, of which more than half (approximately 60%) are proteins. Thus, cellular proteins and protein assemblies occupy a large volume that can have a profound effect on their own native-state stabilities and on their unfolding/refolding rates. In addition, macromolecular crowding can change the properties of a significant fraction of the water in the cell. We review features of the molecular crowding effect which are relevant for describing the microscopic mechanism of thermal injuries.

Explore related subjects

MathematicsModels, MolecularProtein ConformationProtein DenaturationProtein FoldingProteinsThermodynamicsWaterJournal ArticleReview

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Florin Despa, Dennis P Orgill, Raphael C Lee. 2005. Molecular crowding effects on protein stability.. https://doi.org/10.1196/annals.1363.005

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

Content uniformity and assay requirements in current regulations.

The acceptance of a tablet batch is based both on the content uniformity test and on the assay. It is shown that these two characteristics are not independent, and the acceptance criteria for them are not even consistent. For content uniformity range three methods of calculation are compared: the present European Pharmacopoeia method, a tolerance range method with improved k tolerance factor and a one-way random effects analysis of variance model. To resolve the inconsistency several options are discussed: applying the holistic content uniformity range alone; using content uniformity standard deviation and assay mean simultaneously or applying a criterion based on Taguchi's quadratic loss function.

Mathematics↗
PubMed2006

Stable time filtering of strongly unstable spatially extended systems.

Many contemporary problems in science involve making predictions based on partial observation of extremely complicated spatially extended systems with many degrees of freedom and with physical instabilities on both large and small scale. Various new ensemble filtering strategies have been developed recently for these applications, and new mathematical issues arise. Because ensembles are extremely expensive to generate, one such issue is whether it is possible under appropriate circumstances to take long time steps in an explicit difference scheme and violate the classical Courant-Friedrichs-Lewy (CFL)-stability condition yet obtain stable accurate filtering by using the observations. These issues are explored here both through elementary mathematical theory, which provides simple guidelines, and the detailed study of a prototype model. The prototype model involves an unstable finite difference scheme for a convection-diffusion equation, and it is demonstrated below that appropriate observations can result in stable accurate filtering of this strongly unstable spatially extended system.

Mathematics↗