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A general approach for antibody purification utilizing [Protein A-catechol:Fe3+] macro-complexes.

Guy Patchornik·Amnon Albeck
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Abstract

A general platform for antibody purification utilizing free nonimmobilized Protein A modified with the strong metal chelator catechol (ProA-CAT) and Fe3+ ions is presented. The mechanism of purification requires formation and precipitation of macro-complexes composed of [ProA-CAT:IgG:Fe3+]. Target IgGs are eluted directly from the precipitates (i.e. pellets) at pH 3 in high yields (71-80%) and high purity (>95%), without dissociating the [ProA-CAT:Fe3+] insoluble macro-complex.

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Guy Patchornik, Amnon Albeck. A general approach for antibody purification utilizing [Protein A-catechol:Fe3+] macro-complexes.. https://doi.org/10.1021/bc050361%2B

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