A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA).

Mirco Castoldi; Sabine Schmidt; Vladimir Benes; Mikkel Noerholm; Andreas E Kulozik; Matthias W Hentze; Martina U Muckenthaler

doi:10.1261/rna.2332406

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A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA).

Mirco Castoldi·Sabine Schmidt·Vladimir Benes·Mikkel Noerholm·Andreas E Kulozik·Matthias W Hentze·Martina U Muckenthaler

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Abstract

MicroRNAs represent a class of short (approximately 22 nt), noncoding regulatory RNAs involved in development, differentiation, and metabolism. We describe a novel microarray platform for genome-wide profiling of mature miRNAs (miChip) using locked nucleic acid (LNA)-modified capture probes. The biophysical properties of LNA were exploited to design probe sets for uniform, high-affinity hybridizations yielding highly accurate signals able to discriminate between single nucleotide differences and, hence, between closely related miRNA family members. The superior detection sensitivity eliminates the need for RNA size selection and/or amplification. MiChip will greatly simplify miRNA expression profiling of biological and clinical samples.

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Animals Biophysical Phenomena Biophysics Blotting, Northern DNA Probes Gene Expression Profiling Mice Mice, Inbred C57BL MicroRNAs Nucleic Acid Hybridization Nucleic Acids Oligonucleotide Array Sequence Analysis Oligonucleotide Probes Sensitivity and Specificity Journal Article Research Support, Non-U.S. Gov't

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Mirco Castoldi, Sabine Schmidt, Vladimir Benes, Mikkel Noerholm, Andreas E Kulozik, Matthias W Hentze, Martina U Muckenthaler. 2006-03-15. A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA).. https://doi.org/10.1261/rna.2332406

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