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PubMed · 16544620

Diffraction phase microscopy for quantifying cell structure and dynamics.

Gabriel Popescu·Takahiro Ikeda·Ramachandra R Dasari·Michael S Feld
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Abstract

We have developed diffraction phase microscopy as a new technique for quantitative phase imaging of biological structures. The method combines the principles of common path interferometry and single-shot phase imaging and is characterized by subnanometer path-length stability and millisecond-scale acquisition time. The potential of the technique for quantifying nanoscale motions in live cells is demonstrated by experiments on red blood cells.

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Cell MovementCells, CulturedEquipment DesignEquipment Failure AnalysisErythrocytesHumansImage EnhancementImage Interpretation, Computer-AssistedMicroscopy, Phase-ContrastRefractometryReproducibility of ResultsSensitivity and SpecificityJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't

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Gabriel Popescu, Takahiro Ikeda, Ramachandra R Dasari, Michael S Feld. 2006-03-15. Diffraction phase microscopy for quantifying cell structure and dynamics.. https://doi.org/10.1364/ol.31.000775

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