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Polysaccharide microarrays for polysaccharide-platelet-derived-growth-factor interaction studies.

Olivier Carion·Julien Lefebvre·Guy Dubreucq·Latifa Dahri-Correia·José Correia·Oleg Melnyk
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Abstract

Polysaccharide microarrays have great potential for the high-throughput analysis of polysaccharide-protein interactions. Here we demonstrate that a polysaccharide microarray prepared by printing a library of dextran polymers derivatized by methylcarboxylate, benzylamide, and sulfate groups (DMCBSu compounds) on to glass slides permitted the rapid identification of a set of compounds able to interact with the platelet-derived growth factor BB (PDGF-BB) isoform, a growth factor involved in wound healing. Microarray interaction results were compared to the capacity of DMCBSu compounds to potentiate the in vitro PDGF-BB-induced proliferation of human dermal fibroblasts.

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BecaplerminCell ProliferationDextransFibroblastsHumansMicroarray AnalysisMolecular WeightPeptide LibraryPlatelet-Derived Growth FactorPolysaccharidesProto-Oncogene Proteins c-sisSurface PropertiesJournal ArticleResearch Support, Non-U.S. Gov't

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Olivier Carion, Julien Lefebvre, Guy Dubreucq, Latifa Dahri-Correia, José Correia, Oleg Melnyk. 2006. Polysaccharide microarrays for polysaccharide-platelet-derived-growth-factor interaction studies.. https://doi.org/10.1002/cbic.200500387

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