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PubMed · 165549

Modification of surface membrane antigens by trypsin.

J A Molinari·D Platt
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Abstract

Cultured 3T3 and PY-3T3 mouse fibroblasts were subjected to mild treatment with twice crystallized trypsin and their immunogenicity tested in ICR/CD-1 mice. Surface antigenic modifications were observed using the foot pad swelling assay for detection of delayed hypersensitive responses. Mice immunized with trypsin-treated 3T3 cells showed cell-mediated immune reactions following challenge with either homologous antigen or untreated PY-3T3 fibroblasts. In contrast, treatment of the virus-transformed cells with trypsin decreased their antigenicity as determined by this in vivo assay.

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AnimalsAntigensCell MembraneCell Transformation, NeoplasticCells, CulturedFibroblastsHypersensitivity, DelayedImmunity, CellularMiceMice, Inbred ICRPolyomavirusTrypsinComparative StudyJournal Article

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J A Molinari, D Platt. 1975. Modification of surface membrane antigens by trypsin.. https://doi.org/10.3181/00379727-148-38674

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