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Differences between the fingerprints generated from total RNA and poly-A RNA using a modified procedure of cDNA-AFLP and silver staining.

Jian-Rong Guo·F Schnieder·J A Verreet
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Abstract

A modified procedure of cDNA-amplified fragment length polymorphism (cDNA-AFLP) combined with silver staining is described which generates clear band patterns from both fungal total RNA and poly-A RNA. The silver staining procedure takes only 25 min. Some 42-55 transcript-derived fragments (TDFs) were yielded from the total RNA of eight fungal pathogens. In comparison with the fingerprints generated from Mycosphaerella graminicola total RNA, those from poly-A RNA showed eight TDFs with an increased intensity and also seven additional TDFs. It suggests that poly-A RNA is preferable as starting material for ideal cDNA-AFLP analysis.

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Comet AssayDNA FingerprintingDNA, ComplementaryPolymorphism, Restriction Fragment LengthRNA, FungalRNA, MessengerSilver StainingJournal ArticleResearch Support, Non-U.S. Gov't

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Jian-Rong Guo, F Schnieder, J A Verreet. 2006. Differences between the fingerprints generated from total RNA and poly-A RNA using a modified procedure of cDNA-AFLP and silver staining.. https://doi.org/10.1007/s10529-005-5529-9

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