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PubMed · 16565076

New insights into BS69 functions.

Guillaume Velasco·Steve Grkovic·Stéphane Ansieau
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Abstract

The BS69 protein has been commonly described as a co-repressor associated with various transcription factors. However, this hypothesis relied predominantly on overexpression of tagged proteins due to the lack of a reliable BS69 antibody. We present for the first time a complete sequence of BS69 and valuable tools to characterize the endogenous protein. We show that the full-length BS69 protein, as well as minor alternatively spliced isoforms, is ubiquitously expressed, nuclear, and associates with chromatin and mitotic chromosomes. Accordingly, BS69 interacts with a set of chromatin remodeling factors, including ATP-dependent helicases, histone deacetylases, and histone methyltransferases, as well as the E2F6 transcription factor. These data strengthen a role for BS69 in gene repression and link BS69 to chromatin remodeling.

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Alternative SplicingAmino Acid SequenceAnimalsCarrier ProteinsCell Cycle ProteinsCell LineCell NucleusChromatinCo-Repressor ProteinsDNA-Binding ProteinsHistone MethyltransferasesHistone-Lysine N-MethyltransferaseHumansMiceMolecular Sequence DataProtein IsoformsProtein MethyltransferasesUbiquitinJournal ArticleResearch Support, Non-U.S. Gov't

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Guillaume Velasco, Steve Grkovic, Stéphane Ansieau. 2006-03-24. New insights into BS69 functions.. https://doi.org/10.1074/jbc.m600573200

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