Congo red interaction with alpha-proteins.

Jolanta Sereikaite; Vladas-Algirdas Bumelis

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PubMed · 16565748

Congo red interaction with alpha-proteins.

Jolanta Sereikaite·Vladas-Algirdas Bumelis

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Abstract

The ability of Congo red to form complexes with alpha-proteins, human growth hormone and human interferon-alpha2b, was found by absorption difference spectroscopy. A human growth hormone-Congo red complex was isolated by gel-permeation chromatography, and its visible absorption spectrum was registered in comparison to free dye. The ability of Congo red to induce dimerization of human growth hormone was demonstrated using chemical cross-linking agents 1,3,5-triacryloyl-hexahydro-s-triazine and ethylene glycol bis(succinimidylsuccinate).

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Biophysics Chromatography, Gel Congo Red Cross-Linking Reagents Dimerization Fluorescent Dyes Human Growth Hormone Humans Hydrogen-Ion Concentration Interferon alpha-2 Interferon-alpha Protein Binding Recombinant Fusion Proteins Recombinant Proteins Spectrophotometry Succinimides Triazines Journal Article

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Jolanta Sereikaite, Vladas-Algirdas Bumelis. 2006. Congo red interaction with alpha-proteins.. https://pubmed.ncbi.nlm.nih.gov/16565748/

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