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Multifunctional polymeric nanoparticles from diverse bioactive agents.

Paul A Bertin·Julianne M Gibbs·Clifton Kwang-Fu Shen·C Shad Thaxton·William A Russin·Chad A Mirkin·Sonbinh T Nguyen
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Abstract

We present a rational approach for assembling diverse bioactive agents, such as DNA, proteins, and drug molecules, into core-shell multifunctional polymeric nanoparticles (PNPs) that can be internalized in human breast cancer cells. Using ring-opening metathesis polymerization (ROMP), block copolymers containing small-molecule drug segments (>50% w/w) and tosylated hexaethylene glycol segments were prepared and assembled into PNPs that allowed for the surface conjugation of single-stranded DNA sequences and/or tumor-targeting antibodies. The resulting antibody-functionalized particles were readily uptaken by breast cancer cells that overexpressed the corresponding antigens.

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Antibodies, NeoplasmBreast NeoplasmsBridged Bicyclo CompoundsCell Line, TumorDNA, Single-StrandedEthylene GlycolsFluoresceinHeptanesHumansModels, MolecularNanostructuresOligonucleotidesProto-Oncogene Proteins c-bcl-2Erb-b2 Receptor Tyrosine KinasesSpectrophotometry, UltravioletTosyl CompoundsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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Paul A Bertin, Julianne M Gibbs, Clifton Kwang-Fu Shen, C Shad Thaxton, William A Russin, Chad A Mirkin, Sonbinh T Nguyen. 2006-04-05. Multifunctional polymeric nanoparticles from diverse bioactive agents.. https://doi.org/10.1021/ja056378k

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