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Metal-enhanced fluorescence-based RNA sensing.

Kadir Aslan·Jun Huang·Gerald M Wilson·Chris D Geddes
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Abstract

In this communication, we present the proof-of-principle of a new RNA sensing technique, based on metal-enhanced fluorescence, which may improve the potential of solid substrate-based RNA capture assays. In this regard, the detection of RNA is accomplished by annealing a target RNA tagged with a fluorophore to a DNA anchor probe in a single step on a solid surface, where the fluorescence signal is enhanced by silver nanoparticles. Furthermore, the proposed method is highly amenable to high-throughput screening applications and is capable of detecting less than 25 fmol of RNA at a signal-to-noise ratio greater than 20, in several hours compared to that of reverse transcription-PCR at >24 h.

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Biosensing TechniquesFluorescent DyesOligonucleotide ProbesRNARhodaminesSensitivity and SpecificitySilverSpectrometry, FluorescenceJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't

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Kadir Aslan, Jun Huang, Gerald M Wilson, Chris D Geddes. 2006-04-05. Metal-enhanced fluorescence-based RNA sensing.. https://doi.org/10.1021/ja0601179

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