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Exploring human plasma proteome strategies: high efficiency in-solution digestion protocol for multi-dimensional protein identification technology.

Qinhua Cindy Ru·Luwang Andy Zhu·Richard A Katenhusen·Jordan Silberman·Henry Brzeski·Michael Liebman·Craig D Shriver
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Abstract

Multi-dimensional protein identification technology (MudPIT) is becoming a prevalent proteomic approach due to its high-throughput separations and accurate mass detection. Prior to MudPIT analysis, complicated samples required in-solution digestion. Unlike in-gel digestion, in which enzymes work on just a few proteins, in-solution digestion involves simultaneous digestion of hundreds or thousands of proteins. In-solution digestion protocols must therefore be very efficient. Few investigations have evaluated the efficiency of in-solution digestion protocols. The present research compared three such protocols. Results suggest that a protocol utilizing trifluoroethanol (TFE) as denaturant is most efficient.

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Blood ProteinsGene Expression ProfilingHumansProteomeSolutionsJournal ArticleResearch Support, Non-U.S. Gov't

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Qinhua Cindy Ru, Luwang Andy Zhu, Richard A Katenhusen, Jordan Silberman, Henry Brzeski, Michael Liebman, Craig D Shriver. 2005-07-26. Exploring human plasma proteome strategies: high efficiency in-solution digestion protocol for multi-dimensional protein identification technology.. https://doi.org/10.1016/j.chroma.2005.06.080

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