An efficient technique for obtaining sequences flanking inserted retroviruses.

C S Huckaby; R E Kouri; M J Lane; S M Peshick; W T Carroll; S M Henderson; B D Faldasz; P G Waterbury; J N Vournakis

doi:10.1016/1050-3862(91)90024-l

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An efficient technique for obtaining sequences flanking inserted retroviruses.

C S Huckaby·R E Kouri·M J Lane·S M Peshick·W T Carroll·S M Henderson·B D Faldasz·P G Waterbury·J N Vournakis

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Abstract

Genomic mapping studies frequently employ retrovirus-mediated transfer of dominant selectable markers to specific target chromosomes. DNA probes containing sequences adjacent to inserted proviruses are valuable mapping tools in such studies. We have implemented a strategy for amplification of chromosomal sequences flanking the 5' LTR of MoMuLV-based vectors. Probes derived from these amplification products successfully differentiated murine versus human proviral localization in retrovirus-infected mouse-human chromosome 17q hybrid cells.

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Animals Base Sequence Blotting, Southern Cell Line Chromosomes, Human, Pair 17 Cloning, Molecular DNA DNA Probes DNA, Viral Genetic Linkage Genetic Vectors Humans Leukemia Virus, Murine Mice Molecular Sequence Data Polymerase Chain Reaction Proviruses Repetitive Sequences, Nucleic Acid Journal Article

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C S Huckaby, R E Kouri, M J Lane, S M Peshick, W T Carroll, S M Henderson, B D Faldasz, P G Waterbury, J N Vournakis. 1991. An efficient technique for obtaining sequences flanking inserted retroviruses.. https://doi.org/10.1016/1050-3862(91)90024-l

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