Rat brain proteome in morphine dependence.

Anna Bierczynska-Krzysik; Emilia Bonar; Anna Drabik; Marek Noga; Piotr Suder; Tomasz Dylag; Adam Dubin; Jolanta Kotlinska; Jerzy Silberring

doi:10.1016/j.neuint.2006.01.024

PubMed · 16581157

Rat brain proteome in morphine dependence.

Anna Bierczynska-Krzysik·Emilia Bonar·Anna Drabik·Marek Noga·Piotr Suder·Tomasz Dylag·Adam Dubin·Jolanta Kotlinska·Jerzy Silberring

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Abstract

The aim of this study was to reveal potential markers associated with drug dependence, using the proteomic approach. Gels containing samples derived from morphine-treated and control animals were compared and analyzed. Inspection of protein profiles, following TCA/acetone precipitation and the use of nano-scale liquid chromatography coupled to tandem mass spectrometry, allowed for identification of eleven potential dependence markers, mainly cytoplasmic and mitochondrial enzymes, e.g. proteins that belong to GTPase and GST superfamilies, ATPase, asparaginase or proteasome subunit p27 families.

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Animals Brain Chemistry Down-Regulation Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Male Mass Spectrometry Morphine Dependence Nanotechnology Proteome Rats Rats, Wistar Journal Article Research Support, Non-U.S. Gov't

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Anna Bierczynska-Krzysik, Emilia Bonar, Anna Drabik, Marek Noga, Piotr Suder, Tomasz Dylag, Adam Dubin, Jolanta Kotlinska, Jerzy Silberring. 2006-04-03. Rat brain proteome in morphine dependence.. https://doi.org/10.1016/j.neuint.2006.01.024

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