DNA replication during aggregation phase is essential for Myxococcus xanthus development.

Linfong Tzeng; Terri N Ellis; Mitchell Singer

doi:10.1128/jb.188.8.2774-2779.2006

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DNA replication during aggregation phase is essential for Myxococcus xanthus development.

Linfong Tzeng·Terri N Ellis·Mitchell Singer

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Abstract

Previous studies have demonstrated that fruiting body-derived Myxococcus xanthus myxospores contain two fully replicated copies of its genome, implying developmental control of chromosome replication and septation. In this study, we employ DNA replication inhibitors to determine if chromosome replication is essential to development and the exact time frame in which chromosome replication occurs within the developmental cycle. Our results show that DNA replication during the aggregation phase is essential for developmental progression, implying the existence of a checkpoint that monitors chromosome integrity at the end of the aggregation phase.

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Amino Acid Sequence Chromosomes, Bacterial DNA Gyrase DNA Replication DNA, Bacterial Drug Resistance, Bacterial Enzyme Inhibitors Molecular Sequence Data Mutation, Missense Myxococcus xanthus Nalidixic Acid Photomicrography Sequence Alignment Spores, Bacterial Topoisomerase II Inhibitors Journal Article Research Support, N.I.H., Extramural

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Linfong Tzeng, Terri N Ellis, Mitchell Singer. 2006. DNA replication during aggregation phase is essential for Myxococcus xanthus development.. https://doi.org/10.1128/jb.188.8.2774-2779.2006

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