High-throughput RNA interference in functional genomics.

M Janitz; D Vanhecke; H Lehrach

doi:10.1007/3-540-27262-3_5

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High-throughput RNA interference in functional genomics.

M Janitz·D Vanhecke·H Lehrach

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Abstract

RNA interference (RNAi) refers to post-transcriptional silencing of gene expression as a result of the introduction of double-stranded RNA into cells. The application of RNAi in experimental systems has significantly accelerated elucidation of gene functions. In order to facilitate large-scale functional genomics studies using RNAi, several high-throughput approaches have been developed based on microarray or microwell assays. The recent establishment of large libraries of RNAi reagents combined with a variety of detection assays has further improved the performance of functional genome-wide screens in mammalian cells.

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Animals Gene Silencing Genomics Humans RNA Interference RNA, Small Interfering RNA-Induced Silencing Complex Journal Article Review

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M Janitz, D Vanhecke, H Lehrach. 2006. High-throughput RNA interference in functional genomics.. https://doi.org/10.1007/3-540-27262-3_5

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