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A simple zeta method for sperm selection based on membrane charge.

Philip J Chan·John D Jacobson·Johannah U Corselli·William C Patton
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Abstract

OBJECTIVE: The objectives were: [1] to develop a simple zeta potential method for sperm isolation; and [2] to analyze the sperm maturity, morphology, kinematic, and DNA parameters. DESIGN: The phenomenon of sticky sperm adhering to slide surfaces was adapted for collecting charged sperm. SETTING: Clinical and academic research environment. PATIENT(S): Discarded colloid-washed sperm from routine laboratory testing (n = 8). INTERVENTION(S): Sperm were centrifuged in serum-free medium and collected for analyses. MAIN OUTCOME MEASURE(S): Kinematic parameters, DNA integrity, and maturity. RESULT(S): The percentages of mature (73.0% +/- 0.5% vs. control 63.5% +/- 0.5% SEM) and DNA intact sperm (85.0% +/- 0.3% vs. 69.5% +/- 0.5%) increased in the male factor subgroup. Strict normal morphology (19.3% +/- 0.1% vs. 10.0% +/- 0.1%), hyperactivation (7.0% +/- 0.1% vs. 3.6% +/- 0.1%), and progressive motility (29.1% +/- 0.1% vs. 19.9% +/- 0.1%) increased by twofold. CONCLUSION(S): The zeta method improved sperm parameters associated with increased fertilization and pregnancy after assisted reproduction procedures. Manipulation from the attaching-detaching process stimulated sperm metabolism without causing premature acrosome reactions. Total motility was unchanged suggesting a lack of association between total motility and zeta potential.

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Acrosome ReactionCell MembraneCell SeparationDNA FragmentationElectrophysiologyHumansMaleSperm MotilitySpermatozoaJournal Article

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Philip J Chan, John D Jacobson, Johannah U Corselli, William C Patton. 2006. A simple zeta method for sperm selection based on membrane charge.. https://doi.org/10.1016/j.fertnstert.2005.07.1302

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