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A 24-hour screening protocol for identification of vancomycin-resistant Enterococcus faecium.

Steven J Drews·Grant Johnson·Farhad Gharabaghi·Margaret Roscoe·Anne Matlow·Raymond Tellier·Susan E Richardson
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Abstract

We describe a 24-h protocol for the identification of patients who are positive for vancomycin-resistant Enterococcus faecium (VRE), using stool and rectal swab samples and VRE screening broth, automated DNA extraction, and real-time PCR for vanA and vanB genes. Compared to conventional screening methods, this protocol had a high sensitivity and specificity and a negative predictive value.

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AutomationBacterial ProteinsCarbon-Oxygen LigasesEnterococcus faeciumFecesGram-Positive Bacterial InfectionsHumansPolymerase Chain ReactionSensitivity and SpecificityVancomycinVancomycin ResistanceJournal Article

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Steven J Drews, Grant Johnson, Farhad Gharabaghi, Margaret Roscoe, Anne Matlow, Raymond Tellier, Susan E Richardson. 2006. A 24-hour screening protocol for identification of vancomycin-resistant Enterococcus faecium.. https://doi.org/10.1128/jcm.44.4.1578-1580.2006

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