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PubMed · 16622409

Carbohydrate recognition by Clostridium difficile toxin A.

Antonio Greco·Jason G S Ho·Shuang-Jun Lin·Monica M Palcic·Maja Rupnik·Kenneth K-S Ng
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Abstract

Clostridium difficile TcdA is a large toxin that binds carbohydrates on intestinal epithelial cells. A 2-A resolution cocrystal structure reveals two molecules of alpha-Gal-(1,3)-beta-Gal-(1,4)-beta-GlcNAcO(CH(2))(8)CO(2)CH(3) binding in an extended conformation to TcdA. Residues forming key contacts with the trisaccharides are conserved in all seven putative binding sites in TcdA, suggesting a mode of multivalent binding that may be exploited for the rational design of novel therapeutics.

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Bacterial ToxinsCarbohydrate MetabolismCarbohydratesClostridioides difficileEnterotoxinsHydrogen BondingModels, MolecularProtein BindingProtein Structure, TertiaryJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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Antonio Greco, Jason G S Ho, Shuang-Jun Lin, Monica M Palcic, Maja Rupnik, Kenneth K-S Ng. 2006-04-16. Carbohydrate recognition by Clostridium difficile toxin A.. https://doi.org/10.1038/nsmb1084

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