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Full-field swept-source phase microscopy.

Marinko V Sarunic·Seth Weinberg·Joseph A Izatt
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Abstract

We present a full-field phase microscopy technique for quantitative nanoscale surface profiling of samples in reflection. This technique utilizes swept-source optical coherence tomography in a full-field common path interferometer for phase-stable cross-sectional acquisition without scanning. Subwavelength variations in surface sample features are measured without interference from spurious reflections by processing the interferometric phase at a selected depth plane, providing a 1.3 nm stability for high signal-to-noise ratio surface features. Nanoscale imaging was demonstrated by measuring the location of receptor sites on a DNA assay biochip and the surface topography of erythrocytes in a blood smear.

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Cells, CulturedEquipment DesignEquipment Failure AnalysisErythrocytesHumansImage EnhancementImage Interpretation, Computer-AssistedMicroscopy, ConfocalMicroscopy, Phase-ContrastNanotechnologyTomography, Optical CoherenceEvaluation StudyJournal ArticleResearch Support, N.I.H., Extramural

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Marinko V Sarunic, Seth Weinberg, Joseph A Izatt. 2006-05-15. Full-field swept-source phase microscopy.. https://doi.org/10.1364/ol.31.001462

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