Growth arrest and decrease of alpha-SMA and type I collagen expression by palmitic acid in the rat hepatic stellate cell line PAV-1.

Armand Abergel; Vincent Sapin; Nicolas Dif; Christophe Chassard; Claude Darcha; Julie Marcand-Sauvant; Brigitte Gaillard-Martinie; Edmond Rock; Pierre Dechelotte; Patrick Sauvant

doi:10.1007/s10620-005-9031-y

PubMed · 16642426

Growth arrest and decrease of alpha-SMA and type I collagen expression by palmitic acid in the rat hepatic stellate cell line PAV-1.

Armand Abergel·Vincent Sapin·Nicolas Dif·Christophe Chassard·Claude Darcha·Julie Marcand-Sauvant·Brigitte Gaillard-Martinie·Edmond Rock·Pierre Dechelotte·Patrick Sauvant

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Abstract

Liver fibrosis is characterized by an activation of hepatic stellate cells (HSC). During primary culture HSC evolve from a quiescent into an activated phenotype which is characterized by alpha-smooth muscle actin (alpha-SMA) up-regulation, increase in cell growth, and extracellular matrix secretion. HSC culture with trans-resveratrol can lead to deactivation of myofibroblast-like HSC. We used an HSC line, PAV-1, to check the role of retinol and palmitic acid in the deactivation process of HSC. Using mass and metabolic-based methods, Western blot and immunocytochemistry assays, we demonstrated that treatment with palmitic acid (75 muM) alone or in combination with retinol (2 muM) significantly decreased cell proliferation and alpha-SMA expression. We also established that the association of both compounds strongly decreased collagen type I expression. Our results suggest the potential use of palmitic acid alone or in combination with retinol to induce HSC deactivation.

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Actins Animals Cell Cycle Cell Line Cell Proliferation Collagen Type I Liver Liver Cirrhosis Palmitic Acid Rats Vitamin A Journal Article

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Armand Abergel, Vincent Sapin, Nicolas Dif, Christophe Chassard, Claude Darcha, Julie Marcand-Sauvant, Brigitte Gaillard-Martinie, Edmond Rock, Pierre Dechelotte, Patrick Sauvant. 2006-04-27. Growth arrest and decrease of alpha-SMA and type I collagen expression by palmitic acid in the rat hepatic stellate cell line PAV-1.. https://doi.org/10.1007/s10620-005-9031-y

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