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Experimental design considerations in quantification experiments by using the internal standard technique: cholesterol determination by gas chromatography as a case study.

Pedro Araujo·Felicia Couillard·Else Leirnes·Kjersti Ask·Annbjørg Bøkevoll·Livar Frøyland
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Abstract

An overview on experimental design in quantification experiments using the internal standard technique is described. Particular emphasis is given to the modelling of the relationship analyte/internal standard and the estimation of the optimal amount of internal standard to be used throughout the analytical concentration range by using a uniform shell design with a minimum number of seven experiments. The described approach is demonstrated in the analysis of cholesterol in various reference materials by gas chromatography (GC), using alpha-5 cholestan as internal standard. The outlined approaches can be applied in the determination of any kind of analyte.

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CholesterolChromatography, GasReference StandardsJournal Article

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Pedro Araujo, Felicia Couillard, Else Leirnes, Kjersti Ask, Annbjørg Bøkevoll, Livar Frøyland. 2006-05-02. Experimental design considerations in quantification experiments by using the internal standard technique: cholesterol determination by gas chromatography as a case study.. https://doi.org/10.1016/j.chroma.2006.03.119

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