Sequential leaf senescence and correlatively controlled increases in xylem flow resistance.

Abstract

In bean, Phaseolus vulgaris L. (Contender), the directly measured hydraulic resistance of the xylem pathway between roots and primary leaf pulvinal junctions increased rapidly and progressively from 21 to 28 days after planting. These increases in xylem resistance (+390%) were specifically located in the pulvinal junction of the primary leaf. Moreover, they occurred just prior to the onset of primary leaf yellowing. Developmental increases in xylem hydraulic flow resistance and stomatal resistance, as well as subsequent primary leaf yellowing, were completely prevented by detopping the shoots above the primary leaves at 21 days. Thus, the onset of these senescence-associated symptoms was correlatively controlled. In short-term investigations of the mechanisms involved, flow between petiole and cut tip of excised leaves was rapidly reduced by infiltration of 20 picomoles of soluble dextran into the xylem. Moreover, imbibition of approximately 120 picomoles of dextran by excised leaves increased stomatal resistances. A programmed secretion of hormonal concentrations of similar polysaccharides into specific xylem sections in vivo might provide a mechanism for regulating the partitioning of essential xylem supplies between leaves, thus inducing sequential leaf senescence.