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Microarray analysis in drug discovery and clinical applications.

Siqun Wang·Qiong Cheng
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Abstract

DNA microarray analyzes genome-wide gene expression patterns and is used in many areas including drug discovery and clinical applications. This chapter summarizes some of these applications such as identification and validation of anti-infective drug target, study mechanisms of drug action and drug metabolism, classification of different types of tumors, and use of molecular signatures for prediction of disease outcome. A step-by-step protocol is provided for sample preparation, sample labeling and purification, hybridization and washing, feature extraction, and data analysis. Important considerations for a successful experiment are also discussed with emphasis on drug discovery and clinical applications. Finally, a clinical study is presented as an example to illustrate how DNA microarray technology can be used to identify gene signatures, and to demonstrate the promise of DNA microarray as a clinical tool.

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Computational BiologyGene Expression ProfilingHumansMicroarray AnalysisJournal ArticleReview

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Siqun Wang, Qiong Cheng. 2006. Microarray analysis in drug discovery and clinical applications.. https://doi.org/10.1385/1-59259-964-8%3A49

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