A validated chiral CE method for Frovatriptan, using cyclodextrin as chiral selector.

Muzaffar Khan; Balaji Viswanathan; D Sreenivas Rao; G Sreenivas Reddy

doi:10.1016/j.jpba.2006.03.018

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A validated chiral CE method for Frovatriptan, using cyclodextrin as chiral selector.

Muzaffar Khan·Balaji Viswanathan·D Sreenivas Rao·G Sreenivas Reddy

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Abstract

A cyclodextrin modified capillary zone electrophoretic method has been developed for the evaluation of chiral purity of Frovatriptan using sulfobutyl ether beta cyclodextrin (SB-beta-CD) as the chiral selector. The method is highly specific, accurate and reproducible. The method was optimized with a systematic method development approach by optimizing the pH of electrolyte, attempting the separation in different classes of chiral selectors and modifying parameters such as cyclodextrin concentration and the organic modifier type and concentration. The optimized method was validated for specificity, precision, linearity, accuracy and stability in solution using Imidazole as the internal standard. The limit of detection (LOD) and limit of quantification (LOQ) were 1.0 microg/mL and 5.0 microg/mL respectively for each isomer. The method was applied for estimating the chiral purity of various batches of Frovatriptan.

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Carbazoles Cyclodextrins Electrophoresis, Capillary Hydrogen-Ion Concentration Serotonin Receptor Agonists Stereoisomerism Tryptamines Journal Article

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Muzaffar Khan, Balaji Viswanathan, D Sreenivas Rao, G Sreenivas Reddy. 2006-05-02. A validated chiral CE method for Frovatriptan, using cyclodextrin as chiral selector.. https://doi.org/10.1016/j.jpba.2006.03.018

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