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PubMed · 16673924

Chloroplast transformation.

Xiao-Mei Lu·Wei-Bo Yin·Zan-Min Hu
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Abstract

In this chapter we briefly review the developmental history and current research status of chloroplast transformation and introduce the merits of chloroplast transformation as compared with the nuclear genome transformation. Furthermore, according to the chloroplast transformation achieved in oilseed rape (Brassica napus), we introduce the preparation of explants, transformation methods, system selection, identification methods of the transplastomic plants, and experimental results. The technical points, the bottleneck, and the further research directions of the chloroplast transformation are discussed in the notes.

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Biological AssayBlotting, SouthernBrassica rapaCell Culture TechniquesChloroplastsGene ExpressionGene Transfer TechniquesGenetic VectorsPlant LeavesPlants, Genetically ModifiedTransformation, GeneticJournal ArticleResearch Support, Non-U.S. Gov't

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Xiao-Mei Lu, Wei-Bo Yin, Zan-Min Hu. 2006. Chloroplast transformation.. https://doi.org/10.1385/1-59259-959-1%3A285

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